Journal: Biomedicines

Article Title: Comparison of Extracorporeal Shockwave Therapy with Non-Steroid Anti-Inflammatory Drugs and Intra-Articular Hyaluronic Acid Injection for Early Osteoarthritis of the Knees

doi: 10.3390/biomedicines10020202

Figure Lengend Snippet: The results of blood tests between three groups before and after treatment.

Article Snippet: The serum levels of cartilage oligometric protein (COMP) (DCMP0, R&D Systems, Minneapolis, MN, USA), C-telopeptide of type II collagen (CTX-II) (CSB-E14328h, Cusabio Biotech Co., College Park, MD, USA), alkaline phosphatase (ALK-p) (DY370-05, R&D Systems, Minneapolis, MN, USA), osteocalcin (BMS2020INST, Gibco Invitrogen, Carlsbad, CA, USA), and insulin-like growth factor 1 (IGF-1) (DG100B, R&D Systems, Minneapolis, MN, USA) were measured by ELISA kit.

Techniques:

Journal: Molecular Medicine Reports

Article Title: Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate-induced osteoarthritis rats

doi: 10.3892/mmr.2017.7075

Figure Lengend Snippet: Effects of MF on the production and expression of COMP and CTX-II in MIA-induced OA rats. (A) COMP and (B) CTX-II production was measured in the serum of MIA-induced OA rats using commercial ELISA kits. (C) Immunohistochemical staining was used to identify the expression of COMP and CTX-II in the articular cartilage. Data are expressed as the mean ± standard deviation (n=8). *P<0.05, **P<0.01. MIA, monosodium iodoacetate; MF, Mori folium; CLX, celecoxib; COMP, cartilage oligomeric matrix protein; CTX-II, C-telopeptide of type II collagen.

Article Snippet: The primary antibodies against the following proteins were used: MMP-13 (cat no. ab39012; dilution, 1:50; Abcam, Cambridge, UK), cartilage oligomeric matrix protein (COMP; cat no. ab74524; dilution, 1:50; Abcam), C-telopeptide of type II collagen (CTX-II; cat no. PAA686Hu01; dilution, 1:50; Cloud-Clone Corp., Houston, TX, USA), inducible nitric oxide (NO) synthase (iNOS; cat no. sc-651; dilution 1:100; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), and cyclooxygenase-2 (COX-2; cat no. sc-514489; dilution 1:100; Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Immunohistochemical staining, Staining, Standard Deviation

Journal: Bioengineered

Article Title: Silence of TANK-binding kinase 1 (TBK1) regulates extracellular matrix degradation of chondrocyte in osteoarthritis by janus kinase (JAK)-signal transducer of activators of transcription (STAT) signaling

doi: 10.1080/21655979.2021.2018976

Figure Lengend Snippet: Silencing of TBK1 inhibited ECM degradation in OA mice. ACLT mice were infected with lentivirus shTBK1 or shNC. (a) TBK1 mRNA level was detected by qRT-PCR. (b) Safranin O-fast green staining and (c) OARSI score were used for elevating cartilage destruction. (d) Serum CTX-II and (e) COMP were analyzed by the corresponding ELISA kits. * p < 0.05.

Article Snippet: The serum levels of cartilage metabolic markers, including C-terminal crosslinking telopeptide of type II collagen (CTX-II) and cartilage oligomeric matrix protein (COMP), were measured using the corresponding enzyme-linked immuno sorbent assay (ELISA) kits (Wuhan Huamei Biotech Co. Ltd., Wuhan, China).

Techniques: Infection, Quantitative RT-PCR, Staining, Enzyme-linked Immunosorbent Assay